A Comparative Study of Modified Materials of Acetylcholinesterase Biosensor

Abstract : In this study, multi-walled carbon nanotubes (MWCNTs), gold nanoparticles (GNPs) and Prussian Blue (PB) were used for modifying glassy carbon working electrode (GCE) to construct acetylcholinesterase (AChE) biosensor respectively. Chitosan membrane was used for immobilizing AChE through glutaraldehyde cross-linking attachment to recognize pesticides selectively. Before the detection, the enzyme membrane was quickly fixed on the surfaces of modified electrode with O-ring to prepare an ampero-metric acetylcholinesterase biosensor for organophosphate pesticides. The fabrication procedures were characterized by cyclic voltammetry and amperometric i-t curve. The electrochemical behaviours of three modified sensors were compared, and the results showed that AChE-PB/GCE possessed higher oxidation peak current at a lower potential. Based on the inhibition of organophosphorus pesticides to the enzymatic activity of AChE, using dichlorvos as model compound, the sensitivity of three modified biosensors were compared, the results showed that the detection limit of AChE-PB/ GCE was lowest.
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Communication dans un congrès
Daoliang Li; Yande Liu; Yingyi Chen. 4th Conference on Computer and Computing Technologies in Agriculture (CCTA), Oct 2010, Nanchang, China. Springer, IFIP Advances in Information and Communication Technology, AICT-344 (Part I), pp.16-24, 2011, Computer and Computing Technologies in Agriculture IV. 〈10.1007/978-3-642-18333-1_3〉
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Xia Sun, Xiangyou Wang, Wenping Zhao, Shuyuan Du, Qingqing Li, et al.. A Comparative Study of Modified Materials of Acetylcholinesterase Biosensor. Daoliang Li; Yande Liu; Yingyi Chen. 4th Conference on Computer and Computing Technologies in Agriculture (CCTA), Oct 2010, Nanchang, China. Springer, IFIP Advances in Information and Communication Technology, AICT-344 (Part I), pp.16-24, 2011, Computer and Computing Technologies in Agriculture IV. 〈10.1007/978-3-642-18333-1_3〉. 〈hal-01559626〉

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