Whole-Genome Sequencing Reveals Breast Cancers with Mismatch Repair Deficiency

Abstract : Mismatch repair (MMR)–deficient cancers have been discovered to be highly responsive to immune therapies such as PD-1 checkpoint blockade, making their definition in patients, wherethey may be relatively rare, paramount for treatment decisions. In this study, we utilized patterns of mutagenesis known as mutational signatures, which are imprints of the mutagenic processes associated with MMR deficiency, to identify MMR-deficient breast tumors from a whole-genome sequencing dataset comprising a cohort of 640 patients. We identified 11 of 640 tumors as MMR deficient, but only 2 of 11 exhibited germline mutations in MMR genes or Lynch Syndrome. Two additional tumors had asubstantially reduced proportion of mutations attributed to MMR deficiency, where the predominant mutational signatures were related to APOBEC enzymatic activity. Overall, 6 of 11 ofthe MMR-deficient cases in this cohort were confirmed genetically or epigenetically as having abrogation of MMR genes. However, IHC analysis of MMR-related proteins revealed all but one of 10 samples available for testing as MMR deficient.Thus, the mutational signatures more faithfully reported MMR deficiency than sequencing of MMR genes, because they represent a direct pathophysiologic readout of repair pathway abnormalities. As whole-genome sequencing continues to become more affordable, it could be used to expose individually abnormal tumors in tissue types where MMR deficiency has been rarely detected, but also rarely sought
Type de document :
Article dans une revue
Cancer Research, American Association for Cancer Research, 2017, 77 (18), pp.4755-4762. 〈10.1158/0008-5472.CAN-17-1083〉
Liste complète des métadonnées

Littérature citée [29 références]  Voir  Masquer  Télécharger

https://hal.inria.fr/hal-01599736
Contributeur : Marie-France Sagot <>
Soumis le : lundi 2 octobre 2017 - 14:41:05
Dernière modification le : mercredi 11 avril 2018 - 01:58:18

Fichier

4755.full.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Helen Davies, Sandro Morganella, Colin A. Purdie, Se Jin Jang, Elin Borgen, et al.. Whole-Genome Sequencing Reveals Breast Cancers with Mismatch Repair Deficiency. Cancer Research, American Association for Cancer Research, 2017, 77 (18), pp.4755-4762. 〈10.1158/0008-5472.CAN-17-1083〉. 〈hal-01599736〉

Partager

Métriques

Consultations de la notice

154

Téléchargements de fichiers

110