In vitro oxidized HDL and HDL from type 2 diabetes patients have reduced ability to efflux oxysterols from THP-1 macrophages

Yinan Chen 1 Maud Arnal-Levron 1 Françoise Hullin-Matsuda 2, 1 Carole Knibbe 3, 1 Philippe Moulin 1, 4 Céline Luquain-Costaz 1 Isabelle Delton-Vandenbroucke 1
3 BEAGLE - Artificial Evolution and Computational Biology
LBBE - Laboratoire de Biométrie et Biologie Evolutive - UMR 5558, Inria Grenoble - Rhône-Alpes, LIRIS - Laboratoire d'InfoRmatique en Image et Systèmes d'information
Abstract : Oxidized LDL (OxLDL) that are enriched in products of lipid peroxidation including oxysterols have been shown to induce cellular oxidative stress and cytotoxicity therefore accelerating atheroma plaque formation. Upon oxLDL exposure of THP-1 macrophages, intracellular oxidation of LDL derived-cholesterol as well as endogenous cholesterol was increased. The oxysterols intracellularly produced were efficiently exported to HDL whereas apolipoprotein A1 was inefficient. These findings prompted us to investigate the consequences of modification of HDL by oxidation and glycation as observed in type 2 diabetes with respect to oxysterol and cholesterol efflux. We show that efflux of oxysterols was significantly impaired after in vitro oxidation and glycoxidation of HDL whereas glycation alone had no impact. Cholesterol efflux was only slightly decreased by oxHDL or glycoxidized HDL and not changed with glycated HDL. The defect of HDL towards oxysterol efflux was also observed with HDL isolated from diabetic subjects as compared to healthy controls. These findings support a deleterious cellular retention of oxysterols due to dysfunctional HDL in type 2 diabetes.
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Submitted on : Wednesday, December 12, 2018 - 3:08:49 PM
Last modification on : Wednesday, February 19, 2020 - 2:40:17 PM

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Yinan Chen, Maud Arnal-Levron, Françoise Hullin-Matsuda, Carole Knibbe, Philippe Moulin, et al.. In vitro oxidized HDL and HDL from type 2 diabetes patients have reduced ability to efflux oxysterols from THP-1 macrophages. Biochimie, Elsevier, 2018, 153, pp.232-237. ⟨10.1016/j.biochi.2018.04.018⟩. ⟨hal-01952968⟩

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