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Master thesis

Régulation par les miARNs des gènes régulant la fécondité et le développement embryonnaire précoce chez le poisson médaka

Fanny Casse Encadrée 1
1 Dyliss - Dynamics, Logics and Inference for biological Systems and Sequences
Inria Rennes – Bretagne Atlantique , IRISA-D7 - GESTION DES DONNÉES ET DE LA CONNAISSANCE
Abstract : Oogenesis is based on highly regulated and coordinated biological processes involving gene interactions and gene regulation. During oogenesis, ovarian somatic cells undergo many transcriptional changes to prepare undifferentiated germ cells to form gametes. In this context, the regulatory role of microRNAs (miRNAs) is not well knownin fish. Previous studies have found miRNAs particularly expressed in the ovary, such as miR-202, whose KO causes a decrease in gamete quantity and quality. In order to better understand the molecular networks involved, we studied the transcriptomic profile of medaka ovaries (Oryzias latipes)during the reproductive cycle. After reads mapping, annotations were added to those of the reference genome to predict 1131 new long non-coding RNAs and 539 new messenger RNAs. An analysis of the differential expression(DE)reveals 2412 differentially expressed genes. Clustering identified relevant differential expression profiles suggesting a clear difference between the early stages of oogenesiscycle and later ones. In parallel, the analysis identified 37 miRNAs particularly expressed in germinal tissues and 197 in non-germinal tissues. Their targets were predicted and analyzed, suggesting that genes differentially expressed during oogenesis are preferentially targeted by germinal m iRNAs, in accordance with their regulatory role. Our results suggest that some miRNAs would regulate the expression of their targets differently during the oogenesis cycle.
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Master thesis
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https://hal.inria.fr/hal-02192476
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Submitted on : Tuesday, July 23, 2019 - 6:27:53 PM
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Fanny Casse Encadrée. Régulation par les miARNs des gènes régulant la fécondité et le développement embryonnaire précoce chez le poisson médaka. Bio-informatique [q-bio.QM]. 2019. ⟨hal-02192476⟩

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