Development and qualification of clinical grade decellularized and cryopreserved human esophagi
Résumé
Tissue engineering is a promising alternative to current full thickness circumferential esophageal
replacement methods. The aim of our study was to develop a clinical grade Decellularized Human
Esophagus (DHE) for future clinical applications. After decontamination, human esophagi from
deceased donors were placed in a bioreactor and decellularized with sodium dodecyl sulfate (SDS) and
ethylendiaminetetraacetic acid (EDTA) for 3 days. The esophagi were then rinsed in sterile water and
SDS was eliminated by fltration on an activated charcoal cartridge for 3 days. DNA was removed by
a 3-hour incubation with DNase. A cryopreservation protocol was evaluated at the end of the process
to create a DHE cryobank. The decellularization was efcient as no cells and nuclei were observed in
the DHE. Sterility of the esophagi was obtained at the end of the process. The general structure of the
DHE was preserved according to immunohistochemical and scanning electron microscopy images.
SDS was efciently removed, confrmed by a colorimetric dosage, lack of cytotoxicity on Balb/3T3
cells and mesenchymal stromal cell long term culture. Furthermore, DHE did not induce lymphocyte
proliferation in-vitro. The cryopreservation protocol was safe and did not afect the tissue, preserving
the biomechanical properties of the DHE. Our decellularization protocol allowed to develop the frst
clinical grade human decellularized and cryopreserved esophagus.
Domaines
Biomatériaux
Origine : Publication financée par une institution
