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Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells.

Hossam Murad 1 Philippe Collet 1 Cecile Huin-Schohn 1 Nehmann Al-Makdissy 1 Geraldine Kerjan 2 Alain Chedotal 2 Mireille Donner 1 Marie-Dominique Devignes 3 Philippe Becuwe 1 Hervé Schohn 1 Lionel Domenjoud 1 Michel Dauça 1
1 Proliférateurs de Peroxysomes - EA3446
2 NPA - Neurobiologie des processus adaptatifs
3 ORPAILLEUR - Knowledge representation, reasonning
INRIA Lorraine, LORIA - Laboratoire Lorrain de Recherche en Informatique et ses Applications
Abstract : This study tests the hypothesis that the activators of peroxisome proliferator-activated receptors (PPARs) and 9-cis-retinoic acid receptor (RXR) regulate human semaphorin 6B (Sema6B) gene expression. The human MCF-7 breast adenocarcinoma cell line was chosen because it expresses Sema6B at a high level. The Sema 6B mRNA level was analysed by RT-PCR and the semaphorin 6B protein content was determined using a polyclonal antibody that we have produced and characterized. Treatments with fenofibrate (a PPAR alpha activator) and troglitazone (a PPARgamma ligand) strongly decreased the Sema6B mRNA. The drop in Sema 6B mRNA level and in protein content was more important when the treatment combined the action of fenofibrate or troglitazone and 9-cis-retinoic aid. On the other hand, no significant change was observed in the Sema 6B mRNA and protein levels when the cells were exposed to the combined action of GW610742 (a PPARbeta activator) and 9-cis-retinoic acid. These data suggest that PPARalpha/RXR and PPARgamma/RXR heterodimers are involved in the regulation of Sema6B gene expression and open new perspectives concerning the participation of these nuclear receptors in cell recognition and migration.
Keywords : semaphorins semaphorin 6B peroxisome proliferator peroxisome proliferator-activated receptor 9-cis-retinoic acid receptor MCF-7cells
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Journal articles
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https://hal.inria.fr/inria-00103943
Contributor : Marie-Dominique Devignes <>
Submitted on : Thursday, October 5, 2006 - 3:20:38 PM
Last modification on : Friday, February 26, 2021 - 3:28:05 PM

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  • HAL Id : inria-00103943, version 1

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INRIA | NPA | CNRS | UPMC | LORIA | UNIV-LORRAINE | SORBONNE-UNIVERSITE

Citation

Hossam Murad, Philippe Collet, Cecile Huin-Schohn, Nehmann Al-Makdissy, Geraldine Kerjan, et al.. Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells.. International Journal of Oncology, Spandidos Publications, 2006, 28, pp.977-984. ⟨inria-00103943⟩

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