Skip to Main content Skip to Navigation
Journal articles

High-resolution architecture of the outer membrane of the Gram-negative bacteria Roseobacter denitrificans.

Abstract : Summary The outer membrane of Gram-negative bacteria protects the cell against bactericidal substances. Passage of nutrients and waste is assured by outer membrane porins, beta-barrel transmembrane channels. While atomic structures of several porins have been solved, so far little is known on the supramolecular structure of the outer membrane. Here we present the first high-resolution view of a bacterial outer membrane gently purified maintaining remnants of peptidoglycan on the perisplasmic surface. Atomic force microscope images of outer membrane fragments of the size of approximately 50% of the bacterial envelope revealed that outer membrane porins are by far more densely packed than previously assumed. Indeed the outer membrane is a molecular sieve rather than a membrane. Porins cover approximately 70% of the membrane surface and form locally regular lattices. The potential role of exposed aromatic residues in the formation of the supramolecular assembly is discussed. Finally, we present first structural data of the outer membrane porin from the marine Gram-negative bacteria Roseobacter denitrificans, and we perform a sequence alignment with porins of known structure.
Document type :
Journal articles
Complete list of metadata

https://hal.inria.fr/inria-00436539
Contributor : Szymon Jaroslawski <>
Submitted on : Friday, November 27, 2009 - 12:11:07 AM
Last modification on : Monday, July 12, 2021 - 9:39:09 AM
Long-term archiving on: : Thursday, June 17, 2010 - 6:39:57 PM

File

_Manuscript_Repository.pdf
Files produced by the author(s)

Identifiers

Citation

Szymon Jarosławski, Katia Duquesne, James Sturgis, Simon Scheuring. High-resolution architecture of the outer membrane of the Gram-negative bacteria Roseobacter denitrificans.. Molecular Microbiology, Wiley, 2009, 74 (5), pp.1211-1222 ⟨10.1111/j.1365-2958.2009.06926.x⟩. ⟨inria-00436539⟩

Share

Metrics

Record views

557

Files downloads

730