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A detection-based framework for the analysis of recycling in TIRF microscopy

Abstract : Endocytosis/recycling and exocytosis are mechanisms conserved through evolution allowing cells to communicate with their external medium. In order to study these dynamic processes, the present work proposes a patch-based method for detecting recycling or exocytotic events at the Plasma membrane in fast TIRF microscopy combined with the computation of normalized temporal representations of those events. Evaluation, performed on TIRF sequences showing Transferrin receptor (TfR) recycling, validates a high detection rate fully compatible with an automatic data extraction and analysis of the plasma membrane recycling process.
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Contributor : Charles Kervrann Connect in order to contact the contributor
Submitted on : Monday, November 29, 2010 - 6:25:51 PM
Last modification on : Monday, November 7, 2022 - 2:24:09 PM

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Anatole Chessel, Bertrand Cinquin, Sabine Bardin, Jérôme Boulanger, Jean Salamero, et al.. A detection-based framework for the analysis of recycling in TIRF microscopy. IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2010), IEEE Signal Processing Society, Apr 2010, Rotterdam, Netherlands. pp.1281-1284, ⟨10.1109/ISBI.2010.5490230⟩. ⟨inria-00541102⟩



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