Abstract : Deblurring noisy Poisson images has recently been subject of an increasingly amount of works in many areas such as astronomy or biological imaging. In this paper, we focus on confocal microscopy which is a very popular technique for 3D imaging of biological living specimens which gives images with a very good resolution (several hundreds of nanometers), even though degraded by both blur and Poisson noise. Deconvolution methods have been proposed to reduce these degradations and we focus in this paper on techniques which promote the introduction of explicit prior on the solution. One difficulty of these techniques is to set the value of the parameter which weights the trade-off between the data term and the regularizing term. Actually, only few works have been devoted to the research of an automatic selection of this regularizing parameter when considering Poisson noise so it is often set manually such that it gives the best visual results. We present here two recent methods to estimate this regularizing parameter and we first propose an improvement of these estimators which takes advantage of confocal images. Following these estimators, we secondly propose to express the problem of Poisson noisy images deconvolution as the minimization of a new constrained problem. The proposed constrained formulation is well suited to this application domain since it is directly expressed using the anti log-likelihood of the Poisson distribution and therefore does not require any approximation. We show how to solve the unconstrained and constrained problem using the recent Alternating Direction technique and we present results on synthetic and real data using well-known priors such as Total Variation and wavelet transforms. Among these wavelet transforms, we specially focus on the Dual-Tree Complex Wavelet transform and on the dictionary composed of Curvelets and undecimated wavelet transform.