Experimental investigations on flax and glass fabrics reinforced epoxy specimens, i.e. FFRE and GFRE, submitted to fatigue tests are presented in this paper. Samples having [0/90]3S and [±45]3S stacking sequences, with similar fibre volume fractions have been tested under tension–tension fatigue loading. The specific stress-number of cycles to failure (S–N) curves, show that for the [0/90]3S specimens, FFRE have lower fatigue endurance than GFRE, but the [±45]3S FFRE specimens offer better specific fatigue endurance than similar GFRE, in the studied life range (<2 × 10^6). Overall, the three-stage stiffness degradation is observed in all cases except for [0/90]3S FFRE specimens, which present a stiffening phenomenon of around 2–3% which could be related to the straightening of the microfibrils.