Paramecium swimming and ciliary beating patterns: a study on four RNA interference mutations

Abstract : Paramecium cells swim and feed by beating their thousands of cilia in coordinated patterns. The organization of these patterns and its relationship with cell motility has been the subject of a large body of work, particularly as a model for ciliary beating in human organs where similar organization is seen. However the rapid motion of the cells makes quantitative measurements very challenging. Here we provide detailed measurements of the swimming of Paramecium cells from high-speed video at high magnification, as they move in microfluidic channels. An image analysis protocol allows us to decouple the cell movement from the motion of the cilia, thus allowing us to measure the ciliary beat frequency (CBF) and the spatio-temporal organization into metachronal waves along the cell periphery. Two distinct values of the CBF appear at different regions of the cell: most of the cilia beat in the range of 15 to 45 Hz, while the cilia in the peristomal region beat at almost double the frequency. The body and peristomal CBF display a nearly linear relation with the swimming velocity. Moreover the measurements do not display a measurable correlation between the swimming velocity and the metachronal wave velocity on the cell periphery. These measurements are repeated for four RNAi silenced mutants, where proteins specific to the cilia or to their connection to the cell base are depleted. We find that the mutants whose ciliary structure is affected display similar swimming to the control cells albeit with a reduced efficiency, while the mutations that affect the cilia's anchoring to the cell lead to strongly reduced ability to swim. This reduction in motility can be related to a loss of coordination between the ciliary beating in different parts of the cell.
Keywords : paramecium CBF
Type de document :
Article dans une revue
Royal Society of Chemistry, 2014, pp.11. 〈http://pubs.rsc.org/〉. 〈10.1039/C4IB00181H〉
Liste complète des métadonnées

Littérature citée [27 références]  Voir  Masquer  Télécharger

https://hal.inria.fr/hal-01083480
Contributeur : Julien Diener <>
Soumis le : mardi 18 novembre 2014 - 10:00:16
Dernière modification le : jeudi 10 mai 2018 - 02:01:52
Document(s) archivé(s) le : jeudi 19 février 2015 - 10:20:47

Fichier

Paramecium swimming and ciliar...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Anette Funfak, Cathy Fisch, Hatem Abdel Motaal,, Julien Diener, Laurent Combettes, et al.. Paramecium swimming and ciliary beating patterns: a study on four RNA interference mutations. Royal Society of Chemistry, 2014, pp.11. 〈http://pubs.rsc.org/〉. 〈10.1039/C4IB00181H〉. 〈hal-01083480〉

Partager

Métriques

Consultations de la notice

576

Téléchargements de fichiers

360