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Optimal allocation of bacterial resources in fed-batch reactors

Agustin Gabriel Yabo 1, 2, 3 Jean-Baptiste Caillau 4, 2, 3 Jean-Luc Gouzé 5 
1 BIOCORE - Biological control of artificial ecosystems
CRISAM - Inria Sophia Antipolis - Méditerranée , LOV - Laboratoire d'océanographie de Villefranche, INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement
2 McTAO - Mathematics for Control, Transport and Applications
CRISAM - Inria Sophia Antipolis - Méditerranée
5 BIOCORE - Biological control of artificial ecosystems
CRISAM - Inria Sophia Antipolis - Méditerranée , INRA - Institut National de la Recherche Agronomique, LOV - Laboratoire d'océanographie de Villefranche
Abstract : In bacteria, the mechanisms behind the allocation of resources to different cellular functions (i.e. growth or nutrient uptake) have been tuned to optimally adjust cell composition to changing environments. Simple dynamical models of bacterial growth have been key in predicting these mechanisms through a resource allocation perspective. This approach has also been applied to the production of added-value compounds, to determine how to externally adjust the cellular composition in order to maximize metabolite over biomass synthesis. This paper presents a resource allocation perspective of a fed-batch process, based on a multi-scale mechanistic model considering intracellular concentrations of the main cellular components, as well as the dynamics of the bioreactor. The model presented here is a simple coarse-grained self-replicator model of E. coli, accounting for empirical cellular trade-offs observed in previous experimental works. The problem of maximizing an added-value metabolite of interest is written as an Optimal Control problem, in terms of the internal resource allocation parameter and the feeding rate of the bioreactor. Numerical results are provided to better understand the role of cellular composition in fed-batch processing.
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https://hal.inria.fr/hal-03421358
Contributor : Agustin Gabriel Yabo Connect in order to contact the contributor
Submitted on : Tuesday, November 9, 2021 - 3:52:40 PM
Last modification on : Thursday, November 17, 2022 - 10:36:34 PM
Long-term archiving on: : Thursday, February 10, 2022 - 7:16:26 PM

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Agustin Gabriel Yabo, Jean-Baptiste Caillau, Jean-Luc Gouzé. Optimal allocation of bacterial resources in fed-batch reactors. 2022 European Control Conference (ECC), Jul 2022, London, United Kingdom. pp.1466-1471, ⟨10.23919/ECC55457.2022.9838346⟩. ⟨hal-03421358⟩

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