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Blind deconvolution for thin layered confocal imaging
Abstract : In this paper, we have proposed an Alternate Minimization (AM) algorithm for estimating the Point-Spread Function (PSF) of a Confocal Laser Scanning Microscope (CLSM) and the specimen fluorescence distribution. A 3-D separable Gaussian model is used to restrict the PSF solution space and a constraint on the specimen is used so as to favor the stabilization and convergence of the algorithm. The results obtained from the simulation show that the PSF can be estimated to a high degree of accuracy, and those on real data show better deconvolution as compared to a full theoretical PSF model.
Cited literature [34 references]
https://hal.inria.fr/inria-00395523
Contributor : Praveen Pankajakshan Connect in order to contact the contributor
Submitted on : Monday, June 15, 2009 - 4:51:47 PM
Last modification on : Sunday, May 1, 2022 - 3:14:54 AM
Long-term archiving on: : Thursday, June 10, 2010 - 8:29:18 PM
Contributor : Praveen Pankajakshan Connect in order to contact the contributor
Submitted on : Monday, June 15, 2009 - 4:51:47 PM
Last modification on : Sunday, May 1, 2022 - 3:14:54 AM
Long-term archiving on: : Thursday, June 10, 2010 - 8:29:18 PM
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