Abstract : In this paper, we have proposed an Alternate Minimization (AM) algorithm for estimating the Point-Spread Function (PSF) of a Confocal Laser Scanning Microscope (CLSM) and the specimen fluorescence distribution. A 3-D separable Gaussian model is used to restrict the PSF solution space and a constraint on the specimen is used so as to favor the stabilization and convergence of the algorithm. The results obtained from the simulation show that the PSF can be estimated to a high degree of accuracy, and those on real data show better deconvolution as compared to a full theoretical PSF model.
https://hal.inria.fr/inria-00395523
Contributeur : Praveen Pankajakshan
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Soumis le : lundi 15 juin 2009 - 16:51:47
Dernière modification le : mercredi 31 janvier 2018 - 10:24:04
Document(s) archivé(s) le : jeudi 10 juin 2010 - 20:29:18
