This invention concerns a computer-implemented method for estimating diversity in a sample, which can apply to any sample, and in particular to a sample whom species distribution is unknown. The method of the invention comprises steps essentially involving two components, (a) a migration of a sample comprising molecules on a surface area, whereby molecules migrate on a surface area according to their nucleotide or aminoacid sequence and (b) method steps for the diversity measurement, which are preferably performed through the working of one or several computer program(s).